Capillary Separation and Multicapillary Electrophroesis of DNA Matrices by Fluigent

Topics Covered

Background

Introduction

EMMA - The First Product in Fluigent’s Line

Principle Theories behind Fluigent’s EMMA

What Fluigent’s EMMA has to Offer

The Ease of use of Fluigent’s EMMA

Background

Fluigent is a microfluidic company developing new tools for flow control in micro-channels and genetic testing in capillaries and chips. Fluigent employs state-of-the-art technologies in fluidic, electronics and polymer chemistry, in order to built new tools for your processes.

Introduction

Fluigent is developing original and proprietary matrices for DNA separation by capillary and multicapillary electrophoresis. Compatible with existing commercial instruments, these matrices exist in different versions, for either dsDNA or ssDNA separations. They combine outstanding resolution with dynamic coating capabilities and long capillary lifetime.

EMMA - The First Product in Fluigent’s Line

The first product in our line, EMMA™, is designed for the detection and discovery of unknown mutations.

Enhanced Mismatch Mutation Analysis (EMMA™) is a mutation detection method alternative to sequencing. It combines all the advantages of screening before sequencing strategy (High throughput, great reduction of sequencing costs, high productivity).

Principle Theories behind Fluigent’s EMMA

EMMA™ is based on Heteroduplex Analysis (HDA) by multi-capillary electrophoresis with an unprecedented sensitivity, comparable with this of DHPLC or better depending on the application, thanks to a new proprietary matrix composition, combining sieving and chromatographic effects.

EMMA™ also diagnoses in the same run, at no extra cost, large scale-rearrangements.

EMMA™ saves on the PCR step thanks to size multiplexing.

EMMA™ is ideal for the detection of unknown mutations for a diagnostic use as well as for new mutations discovery.

What Fluigent’s EMMA has to Offer

EMMA™ offers the following advantages:

•        Sensitivity at least equal to dHPLC

•        Easy differenciation polymorphism vs. variant

•        High Throughput (covering up to 1 400 fragments/day with a 16 capillaries sequencer)

•        High PCR throughput due to size multiplexing (384 fragments amplified on a 96 plate PCR)

•        Simultaneous dection of large scale rearrangements and point mutations

The Ease of use of Fluigent’s EMMA

Firstly, there is no prior optimization required. A unique set up condition is used whatever the fragment. It is not sensitive to GC content. The unique set up condition can be used even for GC rich fragments and for fragments with a GC rich region flanked by two AT rich regions. Finally, the ease of interpretation of results as a result of time-saving dedicated analysis software Emmalys.

There is also an increase of mutation detection productivity without affecting your expenses.

Source: Fluigent

For more information on this source please visit Fluigent

 

Date Added: Nov 5, 2007 | Updated: Jun 11, 2013
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