Accuracy of BladeCell Disposable Cuvettes for Particle Size Analysis

By AZoNano

Table of Contents

Introduction
Sample Preparation
Measurement
Results
Summary
About Avid Nano

Introduction

Avid Nano's W130i dynamic light scattering (DLS) system utilises patent pending 5µl BladeCell disposable sample cuvettes and is also compatible with a wide variety of standard, square walled measurement cuvettes. It is essential to ensure that the quality and accuracy of the measurement results comply with the requirements of ISO22412:2008, the International Standard for dynamic light scattering. In this technical note, it is explained as to how the results obtained from a BladeCell comply with the standard and are the equal of traditional square walled disposable cuvettes but use only a small fraction of the sample volume.

Sample Preparation

A preparation of 100nm and 200nm diameter NIST traceable certified size standards (Thermo Scientific 3100A & 3200A) was injected into 4.5ml square walled and 5µl BladeCell disposable cuvettes.

Measurement

The prepared cuvettes were mounted in the temperature-controlled sample holder of the W130i DLS system and stabilized at 20°C. A series of acquisitions based on ISO22412 were obtained for each cuvette type and the averages were calculated.

Results

The mean size measured in each cuvette type was found to be within the specified size range based on ISO standards. The Polydispersity Index values lie below the maximum permitted value of 0.100.

Cuvette Type Certified Mean Dia. Measured Mean Dia. Pd Index (<0.1)
5µl BladeCell 93.1 - 108.1 99.9 0.017
4.5ml Kartell #1960
(100nm nominal diameter) 98.0 0.090
5µl BladeCell 192.1 - 210.1 203.9 0.071
4.5ml Kartell #1960
(200nm nominal diameter) 198.0 0.044

Summary

The W130i DLS system is supplied with 5µl BladeCell disposable cuvettes as a standard. The precision of the measurement results are compliant with ISO22412:2008, the International Standard for dynamic light scattering.

About Avid Nano

Avid Nano offers interesting and innovative new dynamic light scattering devices. Avid Nano pursues the highest performance levels, technology, know-how, consistency and convenience for all its products, used in laboratory applications requiring quick, precise DLS measurements in the nm size range.

This information has been sourced, reviewed and adapted from materials provided by Avid Nano.

For more information on this source, please visit Avid Nano.

Date Added: Nov 19, 2011 | Updated: Jun 11, 2013
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