Reducing Sample Preparation Time for Dynamic Light Scattering Using Disposable Cuvettes

By AZoNano

Table of Contents

Introduction
Comparison of Sample Preparation Times with Traditional Quartz Cuvette and with BladeCell Disposable Cuvette
About Avid Nano

Introduction

The W130i dynamic light scattering (DLS) system from Avid Nano is equipped with unique 5 µl BladeCell disposable sample cuvettes. Conventional, quartz glass cuvettes are used by other DLS systems for low volume work, however it is necessary to clean the expensive (non-disposable) quartz glass cuvettes in between uses.

The cleaning process is not only tedious but also introduces the risk of cross contamination between samples.  This article highlights a study by Avid Nano which demonstrates the convenience of using BladeCell disposable sample cuvettes when compared to conventional quartz glass cuvettes to prepare samples for analysis by DLS.

Comparison of Sample Preparation Times

Sample Preparation Time with Traditional Quartz Cuvette

Sample Preparation Time with BladeCell Disposable Cuvette

Total Sample Preparation time: 240 sec

No. of activities: 20

Total Sample Preparation time: 30 sec

No. of activities: 5

Cuvette Preparation (100 seconds)

Cuvette Preparation (0 seconds)

  • Visual inspection through windows (5 seconds)
  • Introduction of filtered water into cuvette using pipette (10 seconds)
  • Cleaning windows by replacing cap (10 seconds)
  • Insert into sample holder (5 seconds)
  • Monitoring scattering intensity to ensure cleanliness (20 seconds)
  • Removal of cuvette (5 seconds)
  • Water removal using pipette (15 seconds)
  • Drying cuvette utilizing air duster or dry gas (30 seconds)
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Sample Loading (20 seconds)

Sample Loading (15 seconds)

  • Visual inspection (5 seconds)
  • Pipette sample into cuvette (10 seconds)
  • Insert cuvette in holder (5 seconds)
  • Pipette sample into cuvette (10 seconds)
  • Insert cuvette in holder (5 seconds)

Measurement Process (sample dependent)

Measurement Process (sample dependent)

Sample Recovery (15 seconds)

Sample Recovery (15 seconds)

  • Remove cuvette (5 seconds)
  • Recover sample (10 seconds)
  • Remove cuvette (5 seconds)
  • Recover sample and dispose cuvette (10 seconds)

Cuvette Cleaning (105 Seconds)

Cuvette Cleaning (0 seconds)

  • Dispensing detergent solution into cuvette (5 seconds)
  • Flushing cuvette with detergent solution using Pasteur pipette (20 seconds)
  • Removal of detergent solution (10 seconds)
  • Flushing cuvette copiously with running water using Pasteur pipette (30 seconds)
  • Water removal and drying with air duster (30 seconds)
  • Cleaning cuvette windows by replacing cuvette cap (10 seconds)
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With the BladeCell Disposable Cuvette, the total sample preparation time is only 30 seconds and involves only 5 separate activities. The BladeCell Disposable Cuvette eliminates cuvette preparation and cleaning time in the process, which involves:

  • Sample loading time of 15 seconds, of which introduction of sample into cuvette using pipette takes 10 seconds and insertion of cuvette into holder takes 5 seconds
  • Sample recovery time of 15 seconds, of which cuvette removal time is 5 seconds and time for sample recovery and cuvette disposal is 10 seconds.

Avid Nano's BladeCell Disposable Sample Cuvette

About Avid Nano

Avid Nano produce the most exciting and innovative new dynamic light scattering products. Avid Nano pursue the highest levels of performance, know-how, technology, convenience and reliability for all our products, used in laboratory applications requiring fast, accurate DLS measurements in the nano-metre size range.

The W130i dynamic light scattering system is a breakthrough, high sensitivity instrument for size and molecular weight measurement of proteins, bio-molecules and sub-micron particles. The W130i combines ultimate measurement performance with an economical benchtop footprint, rugged durability and supreme user convenience. It's extraordinarily compact dimensions are the result of miniaturized 'diamond geometry' S.A.B.Re fibre optic technology.

This information has been sourced, reviewed and adapted from materials provided by Avid Nano.

For more information on this source, please visit Avid Nano.

Date Added: Feb 12, 2013 | Updated: Jun 11, 2013
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