Micro-Dissection of Brain Tissue Samples for Electrophysiology Experiments

By AZoNano

Table of Contents

Introduction
Sample Preparation with miBot Micromanipulators
Advantages of miBot Manipulators
Conclusion
About Imina Technologies

Introduction

Micrometer precision cutting is essential in biology research applications such as electrophysiology for the isolation of thin slices of tissue to perform sample characterization and cell analysis.

This challenging task is highly complex to perform with conventional manual techniques such as utilizing sharp scalpels, due to the inaccuracy of the dissociation of the layers by hand. Although laser cutting is adequately precise, it is tedious and can change the biological material, such as a boiling effect.

This article discusses the use of two miBot micromanipulators from Imina Technologies for the micro-dissection of a brain slice with a length of 3 mm and width of 500 µm under a Leica MZ16 FA stereomicroscope.

Sample Preparation with miBot Micromanipulators

To prepare samples for electrophysiology experiments, the first miBot, consisting of a 76.2 µm diameter probe that is placed on its vertical axis, was utilized to cut the tissue by back and forth movement. The second miBot equipped with a 10 µm tip radius probe was utilized to smoothly separate the cut piece from the rest of the brain slice. The cut progression of a 300 µm thick parasagittal slice of the rodent brain is illustrated in Figure 1.

Figure 1. Steps in micro-dissection of a brain slice using two miBot micromanipulators.

Cutting of the slice was performed by mounting it on a buffered agarosea and immobilizing it with silver wire impalements to avoid its movement during the cutting.

Advantages of miBot Manipulators

The miBot manipulator can move freely, thus facilitating high precision cutting of large brain samples. Moreover, it was easier to modify the position of the manipulators and their angles of approach within minutes according to the morphology of every sample.

This, in turn, significantly reduced the risk of damage caused by improper maneuvers. The smaller footprint of the miBot proved to be a key advantage as it provided better stability during the cutting process when compared to a larger manipulator.

Conclusion

From the aforementioned advantages, it is evident that Imina Technologies miBot micromanipulators are well suited for brain tissue sample preparation for electrophysiology experiments.

About Imina Technologies

Imina Technologies is a privately held company founded in 2009 to exploit more than ten years of research in high precision robotics at the Swiss Federal Institute of Technology in Lausanne, Switzerland (EPFL).

With many years of experience in precision engineering, micro-robotics and nanomanipulation, Imina's interdisciplinary team is geared up for the needs of the most demanding users. Their unique combination of know-how enables them to propose complete solutions for even the most specific applications.

This information has been sourced, reviewed and adapted from materials provided by Imina Technologies.

For more information on this source, please visit Imina Technologies.

Date Added: Mar 12, 2013 | Updated: Jun 11, 2013
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