Magnetic Silica Beads in Genomic Applications

Topics Covered

MagSi Magnetic Silica Beads for DNA Extraction
Physical Properties of the MagSi Beads for DNA Isolation
Use of Carboxylated Particles in Genomics Applications
MagSi Beads for Nucleic Acid Isolation
     MagSi-DNA order information
     MagSi-DNA Allround Order Information
     MagSi-DNA COOH Order Information
     MagSi-DNA Allround COOH Order Information
About AMSBIO

MagSi Magnetic Silica Beads for DNA Extraction

In the past decades genomics has been developed and segregated into high throughput applications like blood and food diagnostics, very complex assays (e.g. epigenetics analysis) and highly reliable assays needed for forensics.

All those different applications, interests and needs cannot be matched with one single method nor with one single magnetic bead. High throughput applications supported by robotic liquid handling stations typically need magnetic beads with strong magnetic response whereas highly complex, manual DNA extraction applications need magnetic beads with highest DNA capture, DNA selectivity and lowest unspecific binding.

Figure 1. Schematic representation of DNA extraction from blood using MagSi-DNA beads

Physical Properties of the MagSi Beads for DNA Isolation

For this purpose amsbio have developed different types of magnetic beads specifically tailored to your needs. This paper should guide you through the company’s MagSi magnetic particle portfolio specifically designed for the different DNA extraction methods and applications. Table 1 and 2 gives you an overview of the physical properties of the core MagSi particles:

Table 1. Physical properties of the MagSi Beads for DNA extraction

  MagSi-DNA MagSi-DNA allround MagSi-DNA 600
Magnetic force ++++ ++ +
Time in suspension +/- +++ ++++
Accessible surface area ++ +++ +++
Bead density ++++ ++ +
Colour black brown brown

Table 2. Specifications of the MagSi Beads for DNA extraction

  MagSi-DNA MagSi-DNA allround comp. Bead
Mean Size 300 nm 1,2 μm 1 μm
Concentration 300 mg/ml 20 mg/ml 50 mg/ml
Shape spherical spherical n.a.
Particle count/ml 4,6* 1012 1,7* 1010 1,7* 108
Density 4,6 kg/ltr 1,4 kg/ltr 2,5 kg/ltr
Particle surface/ml bead solution 13,04 m2 3,85 m2 5 m2
DNA binding capacity /isolation (20 μl bead solution) up to 8 μg up to 6 μg up to 5 μg
DNA yield/mg bead up to 1,3 μg/mg up to 3,2 μg/mg n.a.
Stability pH 3-11 pH 3-11 pH 4-10

Use of Carboxylated Particles in Genomics Applications

MagSi-DNA and MagSi-DNA allround come in plain silica and carboxylated versions. The plain silica MagSi beads are highly robust (even at significant acidic and basic pH values) whereas the carboxylated MagSi beads show highest DNA binding yields and highest DNA purities. Table 3 lists typical DNA extraction applications and the recommended MagSi Beads.

Table 3. Sample type, application and use of MagSi Beads for DNA extraction.

  MagSi-DNA MagSi-DNA COOH MagSi-DNA allround MagSi-DNA allround COOH
PCR - Sequence clean up + + + ++ ++ + +
DNA concentration + + + ++ ++ + +
gDNA isolation + + + + + + ++ + + ++ + +
Small sample volumes + + + 1) + + + + 1) ++ + + 1) + + + + 1)
Large sample volumes + + 1) + + + + 1) ++1) + + + + 1)
Manual platform + + ++ + + ++ + +
Automated platform + + ++2) + ++2) ++ + + ++ + +
  1. The yield and purity are strongly dependent on the buffer system used. The plain silica MagSi-DNA and MagSi-DNA allround beads are optimized for chaotropic buffer systems, whereas the MagSi-DNA COOH and MagSi-DNA alround COOH beads work optimal with non-chaotropic buffer systems.
  2. Automated platform equipped with shaking device (eg Roche MagnaPure XL); the MagSi-DNA allround is the "out-of-the-box" solution on any platform.

MagSi Beads for Nucleic Acid Isolation

MagSi-DNA order information

Magnetic silica particles specifically designed for nucleic acid isolation for rod type robotic systems. (Dense iron oxide core)

Art. Nr. Product Conc. Size Volume
MD01017 MagSi-DNA 300mg/ml 1.2μm 2ml
MD02017 MagSi-DNA 300mg/ml 1.2μm 10ml
MD03017 MagSi-DNA 300mg/ml 1.2μm 100ml

MagSi-DNA Allround Order Information

MagSi magnetic silica beads with high magnetic content optimized for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for robotic and manual workflow.

Art. Nr. Product Conc. Size Volume
MD01018 MagSi-DNA allround 20mg/ml 300nm 2ml
MD02018 MagSi-DNA allround 20mg/ml 300nm 10ml
MD03018 MagSi-DNA allround 20mg/ml 300nm 100ml

MagSi-DNA COOH Order Information

Magnetic silica particles specifically designed for nucleic acid isolation for rod type robotic systems. (Dense iron oxide core). The carboxylated surface guarantees best results in terms of DNA purity and yield. These beads are specially recommended for high DNA yield applications like isolation of viral DNA, DNA sequencing or PCR clean up.

Art. Nr. Product Conc. Size Volume
MD01019 MagSi-DNA COOH 300mg/ml 1.2μm 2ml
MD02019 MagSi-DNA COOH 300mg/ml 1.2μm 10ml
MD03019 MagSi-DNA COOH 300mg/ml 1.2μm 100ml

MagSi-DNA Allround COOH Order Information

MagSi magnetic silica beads with high magnetic content optimized for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for robotic and manual workflow. The carboxylated surface guarantees best results in terms of DNA purity and yield. These beads are specially recommended for high DNA yield applications like isolation of viral DNA, DNA sequencing or PCR clean up.

Art. Nr. Product Conc. Size Volume
MD01020 MagSi-DNA allround COOH 20mg/ml 300nm 2ml
MD02020 MagSi-DNA allround COOH 20mg/ml 300nm 10ml
MD03020 MagSi-DNA allround COOH 20mg/ml 300nm 100ml

About AMSBIO

AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines.

This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.

Source: AMSBIO.

For more information on this source, please visit AMSBIO.

Date Added: May 17, 2013 | Updated: Jun 11, 2013
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