LED Illumination in Optogenetics Techniques

By AZoNano Staff Writers

Table of Content

Introduction
Experimental Framework
Results and Discussion
Conclusion
About Lumen Dynamics Group Inc

Introduction

Optogenetics techniques employ the power of light to activate or inhibit opsonin expressing cells or molecules sans affecting the surrounding cells.

This method is extensively utilized to activate or inactivate ion pumps and channels within cells to reproduce variations that may take place if the channel function is interrupted owing to environmental or genetic changes.

Experimental Framework

Optogenetics is typically performed using a lamp or laser system, as this system tend to exhibit high powers. In this experiment, an LED light source was tested to see whether it would be able to activate the chloride pump halorhodopsin in embryonic zebrafish.

 

Figure 1. X-Cite® XLED1

For the experiment, a dissection microscope was used on which a glass bottom petridish filled with embryonic fish water was placed. Zebrafish at 19-21 hours post fertilization stage was kept in this embryonic fish water.

Lumen Dynamics’ X-Cite® XLED1 (Figure 1) was used as the LED light source. This light guide was directed toward the fish and a high-speed camera was used to image fish behaviour. 20s pulses of yellow light (525nm LED) were followed by 20s pulses of green light (460nm LED). Using a Fastec camera, fish was imaged for a few minutes during the illumination period and beating was counted manually.

 

Results and Discussion

Illumination with green light did not inhibit the zebrafish tail movements in halorhodopsin expressing fish, while the yellow light prevented the spontaneous beating of the tail. However, control fish maintained the spontaneous movements of tail when illuminated with green or yellow light.

A motor neuron enhancer controlled the halorhodopsin expression. In these experiments, the yellow light from an LED source was able to activate halorhodopsin and prevent motor neuron activity. During zebrafish development, Inhibition of halorphodopsin was made in the period of spontaneous activity.

The yellow light was able to prevent the spontaneous movements of the tail in fish expressing halorhodopsin, but not in control fish. It is apparent that LEDs produce less power which has been the case when they were initially introduced into the imaging market. However, with the advent of advanced LED technology, these systems are now being used in applications where they may not have been proved suitable before.

Conclusion

From the above experiment, it is clear that the X-Cite® XLED1 LED sources can be used for activating the optogenetically controlled chloride pump halorhodopsin. The high-power system was sufficient to activate this pump which needs more energy when compared to other molecular pumps, like channelrhodopsin. The study also demonstrates the reliability of using LED sources in other optogenetics studies.

 

About Lumen Dynamics Group Inc

Lumen Dynamics, an Excelitas Technologies® Company is a global leader in the design and creation of innovative light delivery solutions inspired by close to 30 years of light expertise in Manufacturing and Life Science applications. We are a technology company propelled forward by our commitment to providing customer solutions through the innovative application of light.

Precision bonding and UV curing is at the heart of the company’s manufacturing solutions for electronics/optoelectronics and medical device manufacturing processes, in addition to digital printing; while Life Sciences focuses on illumination and measurement technologies for bioscience research, microscopy and instrumentation. The comprehensive family of Lumen Dynamics’ products includes its renowned brands: OmniCure® and X-Cite®.

This information has been sourced, reviewed and adapted from materials provided by Lumen Dynamics Group Inc.

For more information on this source, please visit Lumen Dynamics Group Inc.

Date Added: Dec 2, 2013 | Updated: Jan 14, 2014
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