Determining Viral Structures in Virology Research with Electron Microscopy

By AZoNano Staff Writers

Topics Covered

Introduction
Benefits of the LVEM5 in Virology Research
Summary
About Delong America

Introduction

The high contrast imaging offered by the LVEM5 from Delong is a great advantage in virology. Generally, electron microscopy enables the study of viral components and structures.

The detection and identification of virus particles is done based on morphology. Normally, a magnification of 50,000 is used. Viruses such as adenovirus, rotavirus, adenovirus, astrovirus, calicivirus and Norwalk-like viruses can be studied. Also viruses such as herpesviruses and papillomaviruses are detected.

Negative staining techniques are used to image these sample types in an electron microscope. While using the LVEM5 in virology applications, this is not the case. Issues with sample contrast are eliminated entirely, enabling the researcher to image unstained viruses or virus like particles. By this, researchers can identify virus features that have been masked by stains in the past.

Benefits of the LVEM5 in Virology Research

Low energy electrons, as found in the LVEM5, interact much more strongly with the sample than high energy electrons of classical TEM. LVEM5 electrons are scattered strongly by organic materials resulting in exceptional differentiation of features. The density difference required in a sample to give a 5% contrast difference at 5kV is a mere 0.074g/cm3.

The increased contrast of LVEM5 results is matched with a quick time-to-image period, high-throughput research. It is possible to operate the LVEM5 as a rapid diagnostic tool in the identification of infectious agents and assisting in pathological research. This makes the LVEM5 a very important tool in the rapid detection of pathogens of public health importance.

The key concerns with electron microscopy in this kind of application are the expenses involved in purchasing and maintaining the facility. This is not the case for the small and relatively in-expensive LVEM5. The versatile LVEM5 electron microscope operates with four distinct imaging modes TEM, SEM, STEM and ED. This enables comprehensive imaging and finer study.

Summary

The LVEM5 is an advanced version of electron microscopes, built on a revolutionary technology platform that combines advanced imaging with unparalleled benchtop convenience. Appropriate contrast is a perennial challenge for higher voltage electron microscopes.

Heavy metal stain application is usually required to give these microscopes something to see. Staining can add that contrast but also contributes to sample damage and the appearance of artifacts. However, no staining whatsoever is required with the LVEM5 Benchtop TEM: the leading brand of Electron Microscopes.

About Delong America

The Delong group of companies operates in the high tech, engineering design, manufacturing and sales sectors and span two continents with operations in Europe and North America.

Our facilities in Brno, Czech republic are specialized in the design and manufacturing of precision components and electron optics and vacuum technologies. In the summer of 2007 Delong will open its new building which will be equipped with the latest in design and precision manufacturing innovations.

This information has been sourced, reviewed and adapted from materials provided by Delong America.

For more information on this source, please visit Delong America.

Date Added: Dec 18, 2013 | Updated: Dec 19, 2013
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