Properties and Applications of Cytodiagnostics’ Gold Nanoparticle Products

By AZoNano Staff Writers

Topics Covered

Introduction
Standard Gold Nanoparticles
     Applications of Standard Gold Nanoparticles
Functionalized and Pegylated Gold Nanoparticles
About Cytodiagnostics

Introduction

Cytodiagnostics applies unique proprietary protocols for producing spherical and non-spherical gold nanoparticles (Figure 1), yielding homogeneously shaped nanoparticles with a narrow size distribution. Through surface modification of gold nanoparticles, Cytodiagnostics provides multifunctional nanoparticles, including antibody gold conjugates, fluorescent gold nanoparticles, carboxyl, biotin, amine and methyl functionalized particles.

Figure 1. Gold nanoparticles in bottles

Standard Gold Nanoparticles

Cytodiagnostics prepares its standard citrate stabilized spherical gold nanoparticles (Figure 2) using a modified proprietary citrate reduction protocol. These nanoparticles are engineered for high protein binding efficiency and are suitable for a variety applications, including surface-enhanced Raman spectroscopy, dark field microscopy, lateral flow, and conjugate development.

With their high shape uniformity, Cytodiagnostics colloidal gold nanoparticles reduce the inconsistency within an assay by facilitating control over the available surface area, while allowing protein absorption to the gold nanoparticles. This leads to higher performance for a specific assay at a lower cost. TEM images of gold nanoparticles are shown in Figure 3.

Figure 2. Gold nanoparticles TEM

Cytodiagnostics supplies Standard Gold Nanoparticles in 0.1mM phosphate buffered saline (PBS) with a diameter range of 5-400nm. They are surface stabilized with citrate. Cytodiagnostics also offers these particles in a surfactant stabilized format.

Applications of Standard Gold Nanoparticles

Here is a selection of sample applications for Cytodiagnostics' Standard Gold Nanoparticles.

  • Surface Enhanced Raman Spectroscopy (SERS)
  • Dark Field Microscopy
  • Electron Microscopy
  • Lateral Flow Assays
  • Biological Sensor Development
  • Immunochromatography
  • Immunostaining
  • Conjugate Development

Functionalized and Pegylated Gold Nanoparticles

Cytodiagnostics supplies highly concentrated functionalized and PEGylated gold nanoparticles with the same narrow size distribution as their non-functionalized particles. The company offers four different types of surface modifications: NHS-ester, carboxyl, amine, methyl (methoxy), and biotin.

The methyl gold nanoparticles offered by Cytodiagnostics do not readily adsorb proteins, as they are more inert than non-functionalized gold nanoparticles. This property makes them suitable for applications involving reduced protein binding.

Cytodiagnostics offers its NHS-activated gold nanoparticles in simple one-step conjugation kit formats. No activation is required for these particles, which can be directly mixed with the protein of interest to form a functional gold conjugate.

The carboxyl gold nanoparticles provided by Cytodiagnostics are ideal for covalent conjugation of proteins. Cytodiagnostics’ amine functional gold nanoparticles are suitable for conjugation of proteins, DNA, and other ligands utilizing standard EDC/NHS conjugation chemistry.

Moreover, the company offers the option of two different lengths of PEG spacers between the gold surface and the functional group to allow customers to select the most appropriate surface functionality for their specific application.

This facilitates optimization of linker length between the surface of gold nanoparticles and the functional group in specific applications of customers.

About Cytodiagnostics

Cytodiagnostics is a biotechnology company based in Burlington, Ontario, Canada. We focus on providing and developing nanotechnology derived products and services for the international life science market. Our goal is to serve our customers with the highest quality products to ensure success in their research and development efforts.

This information has been sourced, reviewed and adapted from materials provided by Cytodiagnostics.

For more information on this source, please visit Cytodiagnostics.

Date Added: Feb 27, 2014 | Updated: Mar 5, 2014
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