Automated, high-throughput dynamic light scattering (DLS) measurements can be done with the DynaPro® Plate Reader II on macromolecular and nanoparticle samples directly in situ in industry-standard 96, 384, or 1536 well microplates, using 5 minutes acquisition time per well and as little as 1.5 µL of sample. Without any sample carryover concerns, these features significantly improve formulation screening for aggregation, particle size, thermal stability (via the unfolding temperature TM or aggregation temperature Tonset), colloidal stability (via the diffusion interaction parameter kD or D1), and even the viscosity of highly concentrated protein solutions.
The DYNAMICS® software for data acquisition, control and analysis offers an API which supports full integration with industry leading liquid handling robotics.
It is possible to load samples in duplicate or triplicate and also scanned a number of time offering the right measure of data reproducibility and repeatability. It is possible to perform DLS measurements in the DynaPro Plate Reader outside the formulation lab in lesser time and without the difficulties of traditional cuvette-based DLS. The DynaPro Plate Reader II also has an integrated camera, which snaps a photo of each microwell for diagnostic purposes, to determine the presence of a precipitate, bubble, or an empty well.
The key features of DynaPro are:
- Sensitivity: 0.125mg/mL lysozyme
- Range of hydrodynamic radius Rh: 0.5 nm to 1000 nm
- Temperature range: 4°C to 85°C
- DYNAMICS software provides a comprehensive set of analyses for macromolecules and nanoparticles