The NS500 uses Nanoparticle Tracking Analysis (NTA) to detect and
visualize populations of nanoparticles in liquids from 10-1000nm,
dependent on material, and measures the size of each particle from
direct observations of diffusion. Additionally, NTA measures
concentration and a fluorescence mode will differentiate
suitably-labelled particles within complex background suspensions. Zeta
potential measurements are similarly particle-specific. It is
this particle-by-particle methodology that takes NTA beyond traditional
light scattering and other ensemble techniques in providing
high-resolution particle size distributions and validates data with
information-rich video files of the particles moving
The goal of the NS500 is to provide an easy-to-use, reproducible
platform applicable for specific and general nanoparticle
characterisation use. So whether the application is to control size in
nanoparticle development, provide rapid virus titer or measure the
kinetics of protein aggregation, the NS500 system is the solution.
The NS500 has fluorescence capability, enabling the user to tune
into individual particles, with sensitivity to detect individual
quantum dots whilst eliminating background interference of other
particles and media. Standard beads may be used to bind to single
particles for optimum study.
The fluid handling capability of NS500 provides the user with auto
sample presentation and in-situ cleaning. It is now a routine process
to clean the cell with the ability to purge, flush and load samples
through user-customisable software. Dilution may also be optimised then
controlled in this way. Ease of use is further enhanced with a
motorised focus function to readily home in on the particles under
study. In direct response to user feedback this is augmented with an
indexed motorised stage, controlled through the software and providing
excellent repeatability in positioning. The temperature control of the
cell offers a broad range (15C to 55C) and programmable temperature
cycling, with rapid attainment of set-point facilitating faster sample
measurement and turnaround.
With the NS500 ZetaSight upgrade the zeta potential of individual
particles in aqueous suspension can be determined. This provides a
detailed view of the particle distribution in terms of electrical
potential and related stability.