Scientists have shown in the laboratory that metal nanoparticles damaged the
DNA in cells on the other side of a cellular barrier. The research, by the University
of Bristol, is published online this week in Nature Nanotechnology.
Scientists have shown in the laboratory that metal nanoparticles damaged the
DNA in cells on the other side of a cellular barrier. The nanoparticles did
not cause the damage by passing through the barrier, but generated signalling
molecules within the barrier cells that were then transmitted to cause damage
in cells the other side of the barrier.
The research was carried out by a team at the University of Bristol and colleagues,
and is published online this week in Nature Nanotechnology.
The team grew a layer of human cells (about 3 cells in thickness) in the lab.
They then used this barrier to examine the indirect effects of cobalt-chromium
nanoparticles on cells that were lying behind this barrier.
The amount of DNA damage seen in the cells behind the protective barrier was
similar to the DNA damage caused by direct exposure of the cells to the nanoparticles.
Dr Patrick Case, senior author on the study, said: “We need to be clear
that our experimental set up is not a model of the human body. The cells receiving
the exposure were bathed in culture media, whilst in the body they might be
separated from the barrier by connective tissue and blood vessels. The barrier
cells were malignant cell line and 3 cells in thickness whilst all barriers
in the body are less thick and of non malignant cells.”
Gevdeep Bhabra, lead author on the paper, said: “Even though this work
was done in the laboratory, our results suggest the existence of a mechanism
by which biological effects can be signalled through a cellular barrier, thus
it gives us insights into how barriers in the body such as the skin, the placenta
and the blood-brain barrier might work.”
Professor Ashley Blom, Head of Orthopaedic Surgery at the University of Bristol,
added: “If barriers in the body do act in this way, then it gives us insight
into how small particles such as metal debris or viruses may exert an influence
in the body. It also highlights a potential mechanism whereby we might be able
to deliver novel drug therapies in the future.”
These findings suggest that the indirect, as well as the direct, effects of
nanoparticles on cells might be important when evaluating their safety.
More information: Nanoparticles can cause DNA damage across a cellular barrier,
by Gevdeep Bhabra, Aman Sood, Brenton Fisher, Laura Cartwright, Margaret Saunders,
William Howard Evans, Annmarie Surprenant, Gloria Lopez-Castejon, Stephen Mann,
Sean A. Davis, Lauren A. Hails, Eileen Ingham, Paul Verkade, Jon Lane, Kate
Heesom, Roger Newson and Charles Patrick Case. Nature Nanotechnology advance
online publication on 5 November 2009.
Posted November 5th, 2009
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