Technologies Inc. (NYSE:A) today introduced a new HPLC-Chip that dramatically
simplifies the analysis of phosphopeptides in proteomics research. The Phosphochip
is used with Agilent HPLC-Chip/MS instrumentation to identify post-translational
modifications (PTMs) as part of regulating biological pathways. This new HPLC-Chip
was co-developed with Professor Albert J.R. Heck's group at Utrecht University
as part of the Netherlands Proteomics Centre research program.
Agilent's Phosphochip is a unique product that combines enrichment and analysis
of phosphopeptides into a single-step LC/MS analysis when used with the Agilent
HPLC-Chip/MS Interface and Agilent 6000 Series Mass Spectrometers. The HPLC-Chip
has a fully integrated microfluidic design that enables routine PTM analysis.
Protein phosphorylation is one of the most important PTM events within mechanisms
that regulate protein function in biological cells.
"Our new HPLC-Chip is the ultimate ease-of-use tool for phosphopeptide
analysis as it has a fully integrated microfluidic design that enables routine
phosphopeptide analysis without the hassles of clogging and plumbing that arise
in conventional nano LC," said Professor Heck.
"Agilent's broad HPLC-Chip portfolio covers a wide range of applications
and enables customers in a unique way. Through relentless innovation, we continue
to simplify the LC/MS workflow for the research community," said Patrick
Kaltenbach, Agilent general manager, Liquid Chromatography.
Agilent pioneered the HPLC-Chip/MS concept in 2005, combining nanoflow HPLC
columns and connecting capillaries and a spray emitter into a re-useable, credit
card-size device. This allowed the advantages of high sensitivity and low-sample
consumption provided by nano LC/MS to be accessible to scientists without the
troublesome setup involving microvalve, fittings and capillary tubing of conventional
Agilent now offers 12 versions of HPLC-Chips. All Agilent HPLC-Chips are compatible
across the entire portfolio of Agilent mass spectrometers for applications that
include, but are not limited to, peptide quantitation, biomarker discovery,
targeted phosphopeptide analysis, glycan and Monoclonal Antibody characterization,
and small molecule DMPK studies. In addition, Agilent's custom chip program
delivers customized solutions to individual needs. Visit www.agilent.com/chem/hplc-chip
for more details.