Carl Zeiss has launched
a Dual Camera Module for its AxioVision image analysis software to improve the
imaging of fast intracellular processes. The new software is aimed at life scientists,
such as cell biologists, virologists and physiologists, and allows the simultaneous
acquisition of images from two cameras and their synchronization within nanoseconds.
Combined with two identical cameras, the Dual Camera Module will control all
aspects of their operation and enable camera parameters, such as exposure time
or contrast, to be set independently. Due to the simultaneous capture of two
separate images, the software allows users to capture more images in any given
timeframe. Furthermore, artifacts that can occur in the sequential capture of
double-stained structures using a single camera are prevented, as are errors
in ratiometric measurements of two emission channels.
The capture of two different wavelengths in two channels will be especially
valuable in the measurement of emission ratio imaging (Indo-1), fast FRET examinations,
and the imaging of cellular transport processes in cell cultures, tissues or
organisms. It will also be an important asset in the simultaneous imaging of
tissue and cell structures using infrared transmitted-light techniques, such
as IR-DIC and fluorescence excitation. The latter is a key requirement for electrophysiological
work in neurobiology.
The Dual Camera Module also eliminates the need to change filters or use emission
filter wheels to visualize two dyes. Additionally, projecting two images on
the sensor of a camera via optical adapters using Dual Camera does not sacrifice
the field of view. The functionality of the AxioVision Physiology recording
and analysis module, in particular, benefits from the possibilities provided
by Dual Camera and it will aid live cell imaging studies when combined with
the Cell Observer HS and Cell Observer SD microscope systems.