The educational website for fluorescence microscopy www.zeiss.com/campus
has been supplemented with sections on Spectral Imaging and Fluorescent Proteins
and now also provides comprehensive information on these topics.
The application photo taken from www.zeiss.com/campus shows examples of spectral imaging and linear unmixing of multiply stained adherent cell cultures with strongly different (a and b) or comparable (c and d) fluorescence intensities.
By visiting the website, interested scientists can learn more about the concepts
of spectral imaging and FRET microscopy. The new fluorescent protein section
explains how these proteins function and how they are best used in fluorescence
microscopy. Furthermore, detailed articles cover topics such as spectral properties,
brightness, phototoxicity and photostability of the various fluorescent proteins
and offer practical advice on their applications in live cell imaging.
The website also comprises interactive tutorials on both topics, e.g. explaining
chromophore formation and reactions of fluorescent proteins such as PA-GFP,
Kaede and Dronpa or showing how linear unmixing works and FRET biosensors function.
At www.zeiss.com/campus, users can also find application image galleries. They
are invited to share their own results in an application library and they can
visit the reference library to find articles on the most important topics in
fluorescence microscopy, including spectral imaging, FRET and fluorescence proteins.
The Online Campus from Carl Zeiss MicroImaging is available as a source of
information to scientists and students. Summary reports and interactive Flash
animations make it easy for users to quickly learn the various techniques. Theory,
technology and applications in fluorescence microscopy are skillfully combined
in animations. The website was developed by Mike Davidson, a renowned microscopy
expert and online teaching pioneer from Florida State University. Other renowned
scientists in the field of fluorescence microscopy already announced that they
will make a contribution to the Online Campus.