Carl Zeiss completed the
installation and acceptance testing of a transmission electron microscope featuring
a new type of optics for the high-resolution imaging of biological specimens
at the Max Planck Institute for Biophysics in Frankfurt.
PACEM is a transmission electron microscope (TEM) designed to image low-contrast
biological specimens. It was specially developed and installed at the Max Planck
Institute for Biophysics (MPI-BP) by optical expert Carl Zeiss in Oberkochen
and researchers from the Macromolecular Complexes excellence cluster in Frankfurt.
PACEM stands for Phase contrast Aberration Corrected Electron Microscope, which
describes a special procedure that enhances even the slightest differences in
contrast in the specimen, thus enabling specimen imaging that can be better
evaluated.
Professor Dr. Werner Kühlbrandt, Director of the Institute for Structural
Biology at the MPI-BP, explains: “The high-contrast, high-resolution imaging
of biological specimens, from a macromolecule down to cells, is becoming increasingly
important in many scientific inquiries. For example, the detailed structure
of proteins delivers a lot of insights into their function. The PACEM system,
which is an outstanding example of the successful cooperation between industry
and research, will deliver the required images in unparalleled quality and thus
provide new insights.”
Alexander Lazar, TEM Development Manager in the Nano Technology Systems (NTS)
Division at Carl Zeiss SMT emphasizes that “PACEM is another example of
our skill in developing innovative and unique subsystems for transmission electron
microscopes and in integrating them into our modularly designed LIBRA 200 TEM
platform. With these considerably enhanced imaging and analytical possibilities,
we offer our customers added value and help them gain new scientific insights.”
PACEM is based on a ZEISS LIBRA 200 TEM with additional optics to correct spherical
aberration (Cs corrector). Another optical system below the Cs corrector, the
Diffraction Magnification Unit (DMU), generates intermediate magnification of
the diffraction image plane in which specially developed phase plates ensure
contrast enhancement. In general, this combination permits the high-contrast
imaging of large objects down to atomic dimensions, which allows the artifact-free,
3D imaging of macromolecules. In addition to the electron-optical subsystems,
a special cooling device surrounding the specimen plays a key role: in order
to make biological specimens visible in a natural, fully hydrated condition,
they are shock frozen (vitrified) at -190 degrees Celsius and cooled in the
microscope to -175 degrees Celsius. The cooling device prevents the specimen
from warming as a result of heat radiation. At the same time, it binds residual
moisture in the vacuum chamber of the microscope, which would otherwise become
ice on the specimen and reduce contrast and make the image worthless.
In addition to the now installed PACEM system, NTS also set up a special TEM
(SESAM) at the Max Planck Institute for Materials Research in Stuttgart last
year and a CRISP TEM at the caesar research center in Bonn in 2007. Both systems
feature highly special subsystems such as the mandolin filter in SESAM, which
enables maximum resolution electron energy loss spectroscopy (EELS). The TEM
developers at NTS are currently working with the University of Ulm on the SALVE
project which is intended to produce maximum resolution (less than 1 Angstrom)
with low accelerating voltage.