A new presentation on the Malvern
Instruments website discusses how the company's Viscotek TDAmax multiple
detection size exclusion chromatography (SEC) system enables protein scientists
to determine the degree of oligomerisation, aggregation, conformation, and conjugation
in protein samples.
By combining data from light scattering, UV, refractive index (RI), and viscometer
detection, the TDAmax delivers efficient measurement of absolute molecular weight
and molecular size, as well as information about protein structure that is simply
not available using more traditional systems. The presentation can be viewed
The Viscotek TDAmax incorporates RI, light scattering and viscometer detectors,
processing all three signals together at each data slice (5 Hz). By incorporating
a combination of both low angle (7°) and right angle (90°) light scattering
detectors, it can measure the absolute molecular weight (Mw) of a sample directly
as it elutes from the column, without extrapolation or any additional modelling.
Molecular weight, intrinsic viscosity and molecular size are determined across
the entire distribution, state-of-the-art software enabling the results to be
visualized graphically for ease of interpretation. The addition of a fourth
detector, a UV or Diode Array UV, adds in compositional analysis - a very
powerful tool for proteins scientists.