The School of Chemistry + Biochemistry at Georgia Institute of Technology
has chosen the NanoSight
LM-20 system to help in the characterisation of dimensional changes of nanogels
used in the development of drug delivery vehicles for macromolecular therapeutics.
Members of the Lyon Group at Georgia Institute of Technology discuss results from the NanoSight LM-20 system. (main user, Michael Smith, is in the striped shirt)
The GIT research group of Professor Andrew Lyon works on the development and
implementation of new, environmentally responsive (or 'smart') materials
for photonic materials, bioanalysis, and biomimetics. The approach the group
is taking is to design hydrogel nanoparticles that undergo large changes in
their chemical, mechanical, optical, or electrical makeup in response to a chemical
stimulus, biomolecular interaction, or electromagnetic field; such materials
can be viewed as amplifiers or sensitisers of the environmental event. As a
result of their environmental sensitivity, nanogels have potential in a number
Principal user is PhD candidate, Michael Smith. He has been very impressed
with the instrument. 'The NanoSight has been an enabling tool for us in
the characterisation of dilute nanogel samples, where DLS fails to produce reliable
correlation data. For instance, in our recent investigations of degradable nanogels,
we used the NanoSight for sizing and number density estimation throughout the
erosion reaction. In these studies, sample concentrations were typically very
low at later stages of the degradation reaction (due to particle loss). Similarly,
the NanoSight is effective at measuring the fractionated samples from Asymmetrical
flow field-flow fractionation (where samples are greatly diluted following separation).'
Smith continued, 'the LM-20 has also been used to characterize a variety
of other particle types in the group in conjunction with other light scattering
and microscopy methods, to provide topological information for various nanoparticles.'
NanoSight's approach, known as Nanoparticle Tracking Analysis (NTA),
has been shown to be ideally suited to both research and process control use.
The system is an extremely powerful nanoscale research and development tool
for looking a broad range of particle types and concentrations.
To learn more about nanoparticle characterisation using NTA, please visit
the company's website and register for the latest issue of NanoTrail, the company's
NanoSight Ltd, of Salisbury, UK, is the world leading provider of instruments
for the optical detection and real time analysis of sub-micron particles. The
Company supplies unique instruments for nanoparticle analysis in the sub-micron
region that go far beyond existing light scattering techniques in the characterisation
of polydispersed systems. NanoSight delivers direct visualisation of individual
nanoscale particles in suspension from which independent quantitative estimation
of particle size, size distribution and concentration are immediately obtained.
In viral titre assessment, NanoSight produces more accurate concentration results
than plaque assay, and delivers them within minutes, validating the results
with a unique real-time image. Founded in 2004, the company currently has more
than 200 systems in service worldwide, having begun commercial sales in 2006.
The Company has a growing base of users worldwide, including BASF, BP GlaxoSmithKline,
Novartis, 3M Corp, Roche, Solvay & Unilever and many universities.