has announced the release of a new protocol for the Access Array™ System
that enables sequencing of amplicon libraries using GS FLX Titanium Series reagents
on the 454 GS FLX sequencing system.
The Access Array integrated fluidic circuit (IFC) automatically generates emPCR-ready
libraries by simultaneously combining 48 samples and 48 primer sets to produce
48 uniquely-barcoded samples per chip for approximately 7 Euro per sample.
Fluidigm's Access Array IFC, when used with a 454 Titanium sequencer, can capture
up to 24 kb of sequence data per sample, or 1.15 MB per IFC.
"The Access Array System has already been adopted by users around the
world for projects focused on research of cancer, miRNA, and population genetics
that require inexpensive sequencing of targeted enrichment regions for hundreds
or thousands of samples," noted Mike Lee, Fluidigm's Senior Director of
Marketing. "The addition of the GS FLX Titanium Series reagents with longer
reads continues to expand the amount of sequence that can be captured using
our Access Array System. The protocol will benefit our mutual customers by producing
more sequence data in the researcher's regions of interest," he added.
Sequencing library preparation for next-generation sequencers is by far the
most time and labor demanding component of the entire next-generation sequencing
process. While necessary for whole genome sequencing studies, the process can
be almost entirely eliminated for targeted re-sequencing projects when using
Fluidigm's Access Array System. By incorporating adaptor sequences into the
primer design, the final PCR product is ready for emulsion PCR as it already
contains the necessary capture sequences.
The 48.48 Access Array IFC is the first chip that features the ability to
recover reaction products automatically. Once sample processing has been completed,
the IFC automatically returns the samples to inlets from which it can be easily
extracted and readied for sequencing.
Fluidigm (www.fluidigm.com) develops, manufactures and markets proprietary
Integrated Fluidic Circuit (IFC) systems that significantly improve productivity
in life science research. Fluidigm's IFCs enable the simultaneous performance
of thousands of sophisticated biochemical measurements in extremely minute volumes.
These "integrated circuits for biology" are made possible by miniaturizing
and integrating liquid handling components on a single microfluidic device (IFC).
Fluidigm's systems, consisting of instrumentation, software and single-use chips,
increase throughput, decrease costs and enhance sensitivity compared to conventional
laboratory systems. Fluidigm products have not been cleared or approved by the
Food and Drug Administration for use as a diagnostic and are only available
for research use.
* Fluidigm, the Fluidigm logo, Topaz, BioMark, Dynamic Array, Digital Array,
Access Array and NanoFlex are trademarks or registered trademarks of Fluidigm
Corporation. All other trademarks are the property of their respective owners.
* 454® is a registered trademark of 454 Life Sciences Corporation. Genome
Sequencer FLX™ is a trademark of 454 Life Sciences