By Cameron Chai
The NanoFabrication Systems Division of NanoInk has announced that its manuscript entitled ‘Sub-cellular scaled multiplexed protein patterns for single cell co-cultures’ has received approval for publication in Analytical Biochemistry.
The article describes the ability of the NanoInk platform based on its patented Dip Pen Nanolithography technology to build multiple proteins’ sub-cellular scaled features and then analyze and manipulate cellular interactions and the function of single cells.
The NLP 2000 System
Co-culture studies are essential for their capability to reproduce the in vivo condition and simplify the analysis of the cell-cell interactions’ impacts on progenitor or stem cell function and variation in disease and normal states. Traditional co-culture method such as extracellular protein micropatterning, membrane separation of cell types and random seeding of multiple cell populations have their limitations due to numerous experimental variables, which cannot be monitored precisely.
Tom Warwick, who serves as NanoFabrication Systems Division’s General Manager stated that NanoInk's tip-based direct protein printing system deposits features at sub-micron scale with nanoscale registry under normal conditions and allows constant and controlled studies of live single cell co-culture.
Dr. John Collins, who is the lead author of the paper and NanoInk’s Application Scientist, stated that utilizing NanoInk's user-friendly desktop nanolithography system, NLP 2000 System, the scientists can pattern numerous extracellular matrix proteins on a sub-cellular sized glass substrate. C2C12 myoblasts and NIH 3T3 fibroblasts were differentially attached at the single cell level to the fibronectin and laminin features, he added.
Warwick further said the future Analytical Biochemistry article will describe NanoInk’s capability to accurately co-culture various cell types. The company believes that its NanoInk systems will assist knowing the behavior of cells and allow various cell-biology applications that demand accurate control of the cellular microenvironment, he added.