Olympus is introducing
a newly expanded line of FluoView® FV1000-MPE multiphoton laser scanning
microscope systems* for deeper observation and imaging in living specimens.
There are now 14 models and configurations available, allowing researchers to
put together the exact system that can both meet their needs now and grow with
them in the future. The systems, which incorporate Olympus' world-renowned optics,
allow unprecedented capabilities and new flexibility for neuroscientists and
biologists who need to image dynamic processes up to several hundred microns
deep in living specimens with minimal bleaching or damage.
Most of the systems rely on an optimally tuned, custom-designed Spectra-Physics
DeepSee™ laser that minimizes the need for higher laser power at the sample
by compensating for group velocity dispersion. The systems can handle applications
using multiple spatial points or regions of interest, imaging using high-speed
toggling on/off of the laser with an acousto-optic modulator (AOM) and, with
their smaller footprint, are designed for easy, turnkey operation. Finally,
the systems can be conveniently upgraded at any time.
All of the FV1000-MPE systems are useful for repeated, long-range or time-lapse
exposures; infrared (IR) stimulation and imaging are available on some of the
versions. High-performance UIS2 and specific multiphoton objectives are available,
including dipping lenses and IR-corrected optics. System set-up is simple, with
just two alignment points required.
"From researchers who require basic IR imaging to those who demand simultaneous
IR imaging, IR stimulation and visible laser imaging, these new systems offer
higher performance and greater flexibility overall than other commercial multiphoton
systems," said Dennis Donley of Olympus America Inc. "What's
more, they have the smallest footprint available, with units as small as about
5-by-5 feet, and they can be upgraded right in the field."
The systems are built around Olympus' widely used FV1000 confocal imaging
platform, with its proprietary SIM scanner. This allows for two lasers to be
used simultaneously in different regions of the specimen. When combined with
multiphoton capability, the microscopes can use two multiphoton lasers simultaneously
for 3D uncaging experiments.