Posted in | News

Viscotek Releases Application Note Describing SELS Technique Very Helpful in GPC Analysis

Viscotek has released an application note that describes a new light scattering technique that could be very helpful in Gel Permeation Chromatography (GPC) applications where the demands of sample solvent and mobile phase are different.

Solvent enhanced light scattering (SELS) in combination with Gel Permeation Chromatography (GPC) allows users-with two different solvents-to separately optimise the sample preparation step and the chromatographic conditions to increase the dn/dc value to get better light scattering responses.

In the application note an example of a fluoropolymer analysis by SELS-GPC is shown. Although the fluoropolymer is quite soluble in Tetrahydofuran (THF) the dn/dc in this solvent is very low and the light scattering response poor. Whilst using a low refractive index solvent like Acetone increases dn/dc the sample is not soluble in Acetone. But by dissolving the sample in THF and injecting the solution into a mobile phase of Acetone (SELS concept) the researchers were able to maximise the accuracy and reliability of the GPC-LS analysis while using a cheap and safe solvent as mobile phase.

Viscotek - a Malvern Instruments Group company is the global leader in Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) detectors, software and systems for the characterisation of natural and synthetic polymers, copolymers, proteins, protein conjugates and nanoparticulate materials. Viscotek's innovative products and technologies are backed in Europe, North America and Asia by superior technical service and analytical support.

For a copy of the application report please contact Viscotek.

Tell Us What You Think

Do you have a review, update or anything you would like to add to this news story?

Leave your feedback
Your comment type
Submit

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.