Magnetic Field Exposure System for In Vitro Time Lapse Imaging

The desire among researchers to perform in vitro time lapse imaging while exposing live cells to an alternating magnetic field has increased over the past decade.

Nanotherics has now conquered the difficulties involved in performing such experiments, and is launching the Live Cell AMF exposure system for in vitro time lapse imaging. The ergonomic design of this setup enables the user to mount it on any microscope compatible with 6/24/96 tissue culture plates.

Live Cell AMF exposure system for in vitro time lapse imaging mounted onto a Leica inverted fluorescence microscope.

Figure 1. Live Cell AMF exposure system for in vitro time lapse imaging mounted onto a Leica inverted fluorescence microscope.

Achievable Frequencies

Capacitance (nF)

Frequencies (kHz)

*600

128.8

*300

182.2

200

223.1

88

336.4

22

672.8

11

951.5

*Optional additional frequencies

Complete connections for the time lapse imaging AMF exposure setup.

Figure 2: Complete connections for the time lapse imaging AMF exposure setup.

Flexible Compatibility

The Live Cell AMF setup can be connected to any magneTherm system, both old and new, which means that all existing users can use this unique setup. The setup enables physiological temperature control and a 5% CO2 atmosphere, so that the cells can grow uninterrupted for the required time scale. The setup allows microscope experiments with AMF exposure at frequencies ranging from 100 kHz to 1 MHz with a field amplitude of up to 20 mT.

Unique additional features also allow researchers to use this setup to perform time lapse imaging with any type of microscope. This is a significant benefit over costly imaging setups that are usually compatible with one specific microscope type.

Specifications

CO2 input

5 %

Recirculating water bath output

320 ml / minute

Temperature stability

+/- 0.2 °C

Highest frequency available with the system

951.5 kHz (13.2 mT) *

Lowest frequency available with the system

128.8 kHz (20 mT) *

Dimensions

127 x 85 x 26 mm

Tissue culture plate

35 mm tissue culture dish

* will slightly change depending on the inductance and capacitance tolerance.

This information has been sourced, reviewed and adapted from materials provided by nanoTherics.

For more information on this source, please visit nanoTherics.

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