The Viscosizer TD from Malvern Panalytical offers ultra-low volume, solution-based molecular size and stability measurement capabilities along with Poiseuille's law-based relative viscosity assessment. It is an automated biophysical characterization tool, which uses Taylor Dispersion Analysis UV area imaging in microcapillary flow. This capability allows for biophysical characterization of target molecules in solution.
The orthogonal technique for conformational stability analysis can be broadened into label-free measurements in highly complex solutions containing molecules of varying sizes from small to protein-based systems.
Taylor Dispersion Analysis is a simple experimental method where a laminar flow of matched buffer is injected with a nanoliter volume sample pulse. The analysis of the time-evolved concentration profile based on the UV absorption enables determination of hydrodynamic radius of solute molecules and molecular diffusion coefficient.
Unlike other measurement techniques, Viscosizer TD can analyze the stability of proteins, peptides and small molecules even in the presence of interferences such as surfactants and excipients. Using its microcapillary flow methodology, the Viscosizer TD performs complementary relative viscosity measurement for the same sample.
The main features of the Viscosizer TD are:
- Convenient to use
- High resolution measurements with high reproducibility at low concentration and low viscosity
- Total sample volume of 35µL required for triplicate measurements for both size and relative viscosity
- UV detection for molecular sizing allows low concentration measurements
- Matched sample buffer renders excipients and surfactants invisible, enabling label-free characterization of target
- Taylor Dispersion Analysis enables characterization of unmatched molecular size range in solution, from small molecule size, peptide size and protein size, and samples with mixtures of these species
- Orthogonal technique for protein stability assay and protein solubility studies
- Allows optimization of measurement sensitivity and molecular selectivity by changing UV wavelength
The major applications of the Viscosizer TD are:
- Advancing understanding of drug behavior at near in vivo conditions
- Conducting biophysical studies of peptide-based therapeutics at low concentration
- Screening for developability of mAbs at early stages
- Optimization of lead candidates in complex buffers during formulation development
- Assessment of Critical Quality Attributes (CQA) for biotherapeutics