DNase Cleavage of Lambda Digest DNA
DNase1 is an enzyme that nonspecifically cleaves DNA. Here, the Cypher VRS was used to monitor the action of DNase1 on lambda digest DNA. Briefly, the DNA was bound to a mica substrate with MgCl2 buffer, and DNase was introduced. Images were obtained in tapping mode at a line rate of 625 Hz with 320×64 pixels using an Olympus AC10 probe for a frame rate of 8.7 frames per second (fps). Beside the movie, we’ve selected three key frames taken over an interval of just 2.5 seconds. In the first image (top), several DNA strands are observed. In the second image (center), note the DNase enzyme bound to one of the overlapping strands (circle). In the third and last image (bottom), this strand has been cleaved at the binding point.
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