Proteins are solids. When heated they do not melt; instead, they decompose
or sublime directly to the gas phase at low pressures. They cannot be converted
into a liquid form unless they are dissolved in a solvent. A team at the University
of Bristol (UK) and the Max Planck Institute of Colloids and Interfaces in Golm
(Germany) has now successfully liquefied a protein without the assistance of
a solvent. As the research team headed by Stephen Mann reports in the journal
Angewandte Chemie,
the trick is to modify the surface of the protein with a polymeric surfactant.
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The researchers used ferritin for their experiments. This large protein serves
animals and plants as a storage material for iron. Ferritin forms a hollow sphere
that can hold thousands of iron ions. Adam Perriman, a researcher in the Mann
lab, attached polymer chains consisting of a polyethylene oxide portion and
a hydrocarbon portion to these iron-containing ferritin spheres. About 240 polymer
chains were attached to every ferritin molecule. A solution of proteins modified
in this way was freeze-dried. The resulting dry powder could be melted to form
a transparent, viscous red liquid that solidified only upon cooling to –50
°C. In the temperature range between 30 and 37 °C the modified protein
is in a liquid-crystalline state, which means the molecules are oriented more
or less uniformly but (at least partly) lack the three-dimensional lattice that
is formed in the crystalline state. At higher temperatures, the modified protein
acts like a normal liquid. It only decomposes at temperatures above 400 °C.
How does the liquefaction work? The surfactant chains on the ferritin surface
keep the protein spheres apart and shield their surfaces. This prevents the
electrostatic attractive forces between polar molecular groups of neighboring
spheres from holding the proteins together in a solid. The spheres are instead
held together by attractive forces between the hydrocarbon ends of the surfactant
chains. These forces are only strong enough to hold the molecules together as
a liquid. Between 30 and 37 °C the surfactant chains arrange themselves
in an ordered pattern, giving the substance liquid-crystalline properties.
“This is a very exciting result with fundamental significance for understanding
liquids comprising nanostructured components,” says Mann. “Also,
it represents a possible way forward to a novel state of biomolecular matter,
and could therefore have a number of important applications, for example in
biomedical and sensor technology.”
Posted August 17th, 2009
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