manufacturers of unique nanoparticle characterization technology,
the work of Professor Hang (Hubert) Yin's group at the University of
at Boulder where they apply Nanoparticle Tracking Analysis (NTA) to
characterize biological nanoparticles such as microvesicles.
Professor Hang "Hubert" Yin with two members of his research group, Leslie-Anne Morton and Jonel Saludes, use the NanoSight LM10 system.
The Yin Research Lab is interested in studying at the interface of
Biology and engineering with a particular focus on structure-based drug
cell signaling, biochemistry, biotechnology development and membrane
The main research goal of the group is to identify and design
sense membrane curvature to better understand protein/peptide-lipid
interactions and potentially create non-invasive probes to detect
extracellular vesicles. Currently, we are studying microvesicles as
biomarkers of tumor progression and cancer metastasis. These
shed into bodily fluids targeting other cells in the body and are vital
Their experimental protocol involves lipid vesicle preparation by
pressure-controlled extrusion through different membrane pore sizes.
lipid vesicle sizes are prepared in order to mimic the size range of
microvesicles that are shed into the extracellular matrix. Following
extrusion, it is important to validate the vesicle size. By using
Tracking Analysis (NTA) technology, the results provide an accurate
quantification of different populations of vesicle sizes present in the
Prior to NTA, the group mostly used dynamic light scattering (DLS)
the sizes of our synthetic lipid vesicles. Speaking on their use of
Professor Yin says "NTA brought several benefits over existing methods.
The detection ranges from 10 - 2000 nm for vesicle sizes, dimensions
our liposome size of interest. Flow cytometry has a lower limit
~200 nm to accurately measure particle sizes so did not reach our lower
requirement while DLS measures the average size of all the particles
the sample rather than accurately distinguish different pools of
often creating a bias towards larger particles."
The group has recently published a paper in the Journal of
Experimentation that used the NTA technology entitledConstant
Extrusion Method for the Preparation of Nano-sized Liposomes
(Leslie A. Morton, Jonel P. Saludes, Hang Yin). To learn more, please