Spectrofluorometers gauge the degree of analyte in a sample by sensing its fluorescence signature, which is based on particular excitation and emission wavelengths of light.
To test a sample, a specific wavelength of light is sent through the sample, which is held inside a cuvette, exciting the analyte in the sample. After excitation, the analyte emits light at a wavelength longer than the excitation wavelength. A detector then gauges the emitted light, shows the fluorescence value and determines the fluorescence signature of the analyte. The amount of analyte in the sample can then be determined.
A spectrofluorometer is often used in biofuels analysis, biotechnology, quality control, medical analysis, materials science, and academia.