SIAL) today announced the global release of the MISSION(R) LentiPlex(TM)
Pooled Human and Murine shRNA Libraries. Using the shRNA collections of The
RNAi Consortium (TRC), the MISSION LentiPlex Pooled shRNA Libraries enable rapid,
genome-wide RNAi screening at the bench-top level in a convenient and powerful,
pooled lentiviral format. As a member of both TRC-1 and TRC-2 collaborations,
Sigma-Aldrich is the exclusive commercial source for access to TRC shRNA libraries
in lentiviral format.
The MISSION LentiPlex Pooled shRNA Libraries were developed from the entire
TRC-1 human and mouse collections of more than 158,000 shRNA constructs targeting
approximately 16,000 human genes and approximately 16,000 mouse genes. Combining
Sigma-Aldrich's high-throughput lentiviral production methodologies with the
pooled library format enables researchers to perform genome-wide RNAi screens
on various cell types, rapidly and efficiently discovering new targets without
a large investment in infrastructure.
"Sigma-Aldrich is committed to providing customers powerful and unique
screening formats that fully leverage the content of TRC," said Tim Fleming,
Director of Global Commercial Marketing at Sigma-Aldrich. "The pooled lentiviral
format of this product is expected to make the discovery potential of the TRC
collection accessible to any researcher with minimal reagent, time or capital
LentiPlex enables researchers to screen for novel phenotypes in relevant cell
lines and study gene function and disease, both in standard cell lines and difficult
cell types such as primary, non-dividing, growth-arrested or terminally differentiated
cells that are typically resistant to standard siRNA transfection or other viral
delivery systems. In addition, Sigma-Aldrich's shRNA libraries include a U6
promoter for shRNA expression in a wide variety of cell lines including embryonic
stem cells. These characteristics mean researchers can now conduct high quality
pooled shRNA screens in the cell lines of their choice.
The MISSION LentiPlex Pooled shRNA Libraries add to Sigma-Aldrich's suite of
MISSION shRNA products, which include the MISSION TRC-1 shRNA human (MISSION
TRC-Hs1.0) and murine (MISSION TRC-Mm1.0) collections of whole genome arrayed
libraries, gene family sets or individually prepared clones in glycerol, purified
DNA or lentiviral formats. For more information on the MISSION LentiPlex Pooled
shRNA Library, please visit http://www.sigma-aldrich.com/lentiplex.