Manipulating tiny objects like single cells or nanosized beads often requires
relatively large, unwieldy equipment, but now a system that uses sound as a
tiny tweezers can be small enough to place on a chip, according to Penn
State engineers.
 | | "Acoustic tweezers" enable flexible on-chip manipulation and patterning of cells using standing surface acoustic waves. Credit: Tony Jun Huang, Jinjie Shi, Penn State |
"Current methods for moving individual cells or tiny beads include such
devices as optical tweezers, which require a lot of energy and could damage
or even kill live cells," said Tony Jun Huang, assistant professor of engineering
science and mechanics. "Acoustic tweezers are much smaller than optical
tweezers and use 500,000 times less energy."
While optical tweezers are large and expensive, acoustic tweezers are smaller
than a dime, small enough to fabricate on a chip using standard chip manufacturing
techniques. They can also manipulate live cells without damaging or killing
them.
Acoustic tweezers differ from eyebrow tweezers in that they position many tiny
objects simultaneously and place them equidistant from each other in either
parallel lines or on a grid. The grid configuration is probably the most useful
for biological applications where researchers can place stem cells on a grid
for testing or skin cells on a grid to grow new skin. This allows investigators
to see how any type of cell grows.
"Acoustic tweezers are not just useful in biology," said Huang.
"They can be used in physics, chemistry and materials science to create
patterns of nanoparticles for coatings or to etch surfaces."
Acoustic tweezers work by setting up a standing surface acoustic wave. If two
sound sources are placed opposite each other and each emits the same wavelength
of sound, there will be a location where the opposing sounds cancel each other.
This location can be considered a trough. Because sound waves have pressure,
they can push very small objects, so a cell or nanoparticle will move with the
sound wave until it reaches the trough where there is no longer movement. The
particle or cell will stop and "fall" into the trough.
If the sound comes from two parallel sound sources facing each other, the troughs
form a line or series of lines. If the sound sources are at right angles to
each other, the troughs form an evenly spaced set of rows and columns like a
checkerboard. Here too, the particles are pushed until they reach the location
where the sound is no longer moving.
The acoustic tweezers are manufactured by fabricating an interdigital transducer
onto a piezoelectric chip surface. These transducers are the source of the sound.
Next, using standard photolithography, microchannels are fabricated in which
a small amount of liquid with the cells or particles can move around freely.
These microchannels were bonded to the chip to create the area for particle
movement.
To test their device, the researchers, who include Jinjie Shi, Daniel Ahmed
and Sz-Chin Steven Lin, graduate students, engineering science and mechanics;
Xiaole Mao, graduate student in bioengineering, and Aitan Lawit, undergraduate
in engineering science and mechanics, used Dragon Green fluorescent polystyrene
beads about 1.9 micrometers in diameter. They then used cows red blood cells
and the single cell bacteria E. coli to test the acoustic tweezers.
"The results verify the versatility of our technique as the two groups
of cells differ significantly in both shape (spherical beads vs. rod-shaped
E. coli) and size," the researchers reported in a recent issue of Lab on
a Chip. They note that the patterning performance is independent of the particle's
electrical, magnetic and optical properties.
"Most cells or particles patterned in a few seconds," said Huang.
"The energy used is very low and the acoustic tweezers should not damage
cells at all. Because they have different properties, the acoustic tweezers
could also separate live from dead cells, or different types of particles."
Acoustic tweezers technology has significant advantages over existing technologies
because of its versatility, miniaturization, power consumption and technical
simplicity. Huang expects it to become a powerful tool for many applications
such as tissue engineering, cell studies, and drug screening and discovery.
Posted August 28th, 2009
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