Cell and development biologists can now use the sophisticated functions of
the Zeiss AxioVision software,
such as the enhancement of fluorescence signals and the suppression of image
noise, to support their FRET investigations. The new features are found in the
physiology module of the AxioVision 4.7 microscopy software and make FRET experiments
easier and simpler.
The FRET software is used to determine the energy transfer portion between
two adjacent protein molecules through fluorescence energy transfer (FRET),
measure the distance between adjacent protein molecules below the microscope
resolution and obtain quantitative temporal and spatial information about the
bonding and interaction between proteins, lipids, enzymes, DNA and RNA in vivo.
It is ideally matched to the motorized Axio Imager or Cell Observer microscope
systems, although it can be to be used with manual fluorescence microscopes.
The software offers numerous additions to existing and new methods of FRET
measurement by using different corrective techniques or acceptor bleaching.
Users can define their own formulas for the tasks to be performed or use pre-programmed
FRET formulas to make FRET experiments even more simple. Furthermore, it is
possible to modify existing FRET formulas and easily create new evaluation techniques
via the formula field.
AxioVision enables the wide range of microscope accessories and software functions
to be used for processing. For instance, up to 140 frames per second are available
with the AxioCam HS and the dual camera module more than doubles the speed of
capture. In addition, all required time series images (donor, acceptor and FRET)
can be captured using the physiology module.