Sandia National Laboratories
will demonstrate a new hyperspectral confocal fluorescence microscope Friday,
Aug. 8 from 9 a.m.-2 p.m. MDT in Bldg. 897 on Kirtland Air Force Base. This
patent-protected and patent-pending technology has been combined with Sandia’s
unique and proprietary multivariate algorithms and software to form a complete
system for the extraction of quantitative image information from hyperspectral
The microscope has been used for multiplexed live-cell imaging at diffraction-limited
spatial resolutions in a variety of biological applications. Included in the
hyperspectral imaging system is spectral image viewing software to view both
the raw image data as well as the results from the multivariate curve resolution
(MCR) analyses, says Dave Haaland, a researcher in Sandia’s Biomolecular
Analysis and Imaging Department.
The hyperspectral microscope uses laser excitation and collects 512 spectral
emission wavelengths at each voxel in the image over the spectral range from
500 to 800 nanometers at a spectral resolution of 1-3 nm and at an imaging rate
of 8,300 spectra/second (with extension to 64,000 spectra/sec in the future).
Sandia’s proprietary MCR software allows very rapid “discovery”
of all emitting fluorescence species in the image and determination of their
relative concentrations throughout the image without any a priori information.
With this new system, many fluorophores can be monitored simultaneously without
cross talk to achieve higher throughput, greater quantitative accuracy, and
“Sandia is seeking to commercialize this technology by partnering with
interested firms through negotiated licensing agreements,” says Brent
Burdick, licensing executive in Sandia’s Licensing and Intellectual Property
Management Department. “Accordingly, the Aug. 8 demonstration is being
held to allow firms interested in commercializing the technology an opportunity
to see first-hand how the microscope works.”
Due to processing requirements, registrations must be received by Friday, July
25. For further information on the microscope or attending the demonstration,
contact Jonathan Gardner at [email protected] or (505) 845-7653.