The Calypso® II interfaces with a Wyatt multi-angle light-scattering detector (DAWN® HELEOS® II or miniDAWN™ TREOS®) for studying biomolecular interactions, without immobilization or labeling, to determine affinity, true molecular stoichiometry and the kinetics of self-assembly, disassociation or aggregation of macromolecular complexes using composition-gradient multi-angle light scattering (CG-MALS).
A typical system could include a concentration detector such as Wyatt's Optilab® T-rEX™ or a 3rd-party on-line UV absorption detector.
The CALYPSO™ software for control, data acquisition and analysis offers a comprehensive suite of association models for studying self-association and hetero-association including high-order oligomerization, simultaneous self- and hetero-association, and multi-valent interactions.
This innovative technology nicely complements surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and other biophysical techniques for characterizing interactions. In addition, it is much faster and simpler than analytical ultracentrifugation.