A Breakthrough in Process Analytical Technology for Production of Nanoparticles

Wyatt Technology’s ultraDAWN™ is the latest advancement in process analytical technology for production of polymers, nanoparticles, and biopharmaceuticals.

Real-Time Multi-Angle Light Scattering

ultraDAWN quantifies multi-angle light scattering and reports the results, such as rms radius (Rg) and weight-average molecular weight (Mw), in real time. Through real-time multi-angle light scattering (RT-MALS), critical quality attributes (CQAs) can be tracked directly to get instant feedback on the quality of both products and processes.

Benefits of RT-MALS

  • Product quality can be maintained with confidence
  • Process development and scale-up can be expedited
  • Yield can be increased and overall time taken until the release of lot can be reduced

Where MALS and PAT Meet

The ultraDAWN is an exclusive member of the DAWN® range of MALS instruments. It does not reside in the analytical laboratory, like other MALS instruments from Wyatt Technology, but instead, it is adapted to production environments and pilot plants. With the help of the OBSERVER™ software, molar mass and size can be calculated and reported 30 times in each minute.

PAT instruments are very popular and usually track process parameters like pressure, temperature, pH, and feedstock consumption. These process parameters are indirectly tied to the properties and quality of products. The ultraDAWN redefines process development with its direct and continuous measurements of the properties of actual products.

The ultraDAWN features a smart user interface that indicates the molar size and mass together with system health diagnostics. It also includes photodetectors at 18 scattering angles and one more to quantify light transmitted via the flow cell. These features allow the ultraDAWN to offer a solution for:

  • Real-time tracking of Rg from 10 to 250 nm, and Mw from 103 to 109 g/mol. With respect to molar mass analysis, the present version of the OBSERVER™ software needs fixed solvent composition and analyte concentration.
  • Triggering critical alarms or process endpoints
  • Instant detection of contamination by particulates

Process Integration Options

  • Incorporation with production control software through OPC
  • Slipstream or process flow rates from 0.1 to 5 mL/minute
  • On-line or in-line configuration

A Breakthrough in Process Analytical Technology for Production of Nanoparticles

Image Credit: Wyatt Technology

Real-Time Operation

In-line

For continuous processes—like chromatographic purification steps or encapsulation of microfluidic drugs—that occur at flows below 5 mL/minute, in-line RT-MALS offers the shortest feedback loop while the whole process stream is being sampled by the ultraDAWN.

On-line

When the process flow is higher than 5 mL/minute, or when processing occurs within a fixed volume like a homogenizer, a bypass stream is sent from the manufacturing process to the ultraDAWN. Here, RT-MALS information is created in near-real-time.

At-line/off-line

For a comprehensive analysis, products can be characterized by methods like FFF-MALS or SEC-MALS. These techniques offer complete and high-resolution distributions of molar size and mass, albeit not in real time. A regular DAWN® MALS instrument is used along with an Eclipse™ field-flow fractionation system or an SEC system.

A Breakthrough in Process Analytical Technology for Production of Nanoparticles

Image Credit: Wyatt Technology

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