DAWN® HELEOS® II SEC-MALS Advanced Light Scattering Instrument from Wyatt Technology

The DAWN® HELEOS® II Multi-Angle Light Scattering detector is an advanced light scattering instrument designed for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

Key Features

The key features of the DAWN® HELEOS® II are:

  • The DAWN® HELEOS® II can be used in batch mode (off-line) or can be connected on-line to an HPLC, HPSEC or AFFF.
  • It uses a 120mW solid-state laser operating at 658 nm and has on-board digital signal processing hardware for up to four external devices – RI and/or UV detectors.
  • The DAWN® HELEOS® II contains an 18-angle light scattering detector for measuring the absolute molecular weight, size, and conformation of macromolecules in solution.
  • It includes an analog output from the 90° detector for interfacing to strip chart recorders.
  • The DAWN® HELEOS® II also includes a 64,000 colour LCD display, depolarization options, and thermostatic control options.
  • It can be operated up to 210° and interfaces with high temperature GPC systems such as the GPC-IR system available from Polymer Char.

Key Specifications

The key specifications of the DAWN® HELEOS® II are:

   
Light Source: 120 mW GaAs linearly polarized laser;
100 mW delivery to the cell
Laser Wavelength: 658 nm
Laser Lifetime: >10,000 hours
Detectors: 18 angles
Detector type: Custom-designed, ultra-quiet hybrid photodiodes with built-in FET transimpedance amplifiers; optional single photon counting module WyattQELS
Detector Resolution: 24 bit
Mobile phase compatibility: All-solvent compatible (aqueous and organic)
Instrument Options: Ambient;
Ultra-High (UHT) to 210°C;
Heated/Cooled (HC) –15°C to 150°C;
WyattQELS dynamic light scattering module;
COMET ultrasonic flow cell cleaning system.
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