The DynaPro®NanoStar® is a cuvette-based dynamic light scattering (DLS) instrument, which is used for analyzing protein solutions, promiscuous inhibitors and all kinds of nanoparticles or macromolecules in solution. Quartz cuvettes afford precise robust measurements even with just 1 µL of solution to accommodate precious and scarce samples while the disposable cuvettes offer a comparable sensitivity with as little as 4µL. True molar mass measurements are offered by a distinct, optimized static light scattering (SLS) channel.
Using the parallel SLS and DLS channels, the NanoStar determines hydrodynamic radius Rh, size distributions, weight-average molar mass, and solution properties that are relevant to sample stability: the second virial coefficient (A2 or B22), the diffusion interaction parameter (kD or D1), and the temperature of protein melting or aggregation onset (TM, Tonset).
The NanoStar’s optical fiber pick-up can be combined with any of Wyatt's multi-angle light scattering (MALS) detectors - the DAWN® HELEOS® II or miniDAWN™ TREOS® - to add on-line DLS measurements in the same detection volume as chromatographic SEC-MALS or FFF-MALS measurements.
The key features of the DynaPro®NanoStar® Dynamic Light Scattering Detector are:
- Sensitivity: 0.1 mg/mL lysozyme
- Range of hydrodynamic radius Rh: 0.2 nm to 2500 nm
- DYNAMICS® software provides a comprehensive set of analyses for macromolecules and nanoparticles
- Range of molar mass: 300 Da to 1 MDa (may vary with molecular composition, size or solvent)
- Temperature range: -15°C to 150°C