The Calypso® II interfaces with a Wyatt multi-angle light-scattering detector (DAWN® HELEOS® IIor miniDAWN™ TREOS®)for studying biomolecular interactions without immobilization or labeling to determine true molecular stoichiometry, affinity, kinetics of self-assembly, disassociation or aggregation of macromolecular complexes using composition-gradient multi-angle light scattering (CG-MALS). A typical system could include a concentration detector such as Wyatt's Optilab® T-rEX™ or a 3rd-party on-line UV absorption detector.
The key features of the Calypso® II Biomolecular Interaction System are:
- The system features the CALYPSO™ software for control, data acquisition and analysis offers a comprehensive suite of association models for studying self-association and hetero-association including high-order oligomerization, simultaneous self- and hetero-association, and multi-valent interactions.
- This innovative technology very well complements surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and other biophysical techniques for characterizing interactions. Furthermore, it is much faster and simpler than analytical ultracentrifugation.