The Eclipse™AF4 is used for challenging fractionation, sizing and separation tasks for naoparticles, macromolecules and colloids. In combination with a Wyatt DAWN® or miniDAWN™ MALS detector and Optilab® T-rEX™ refractive index detector, the Eclipse AF4 provides a versatile solution offering characterization of absolute molar mass and size and also conformation and conjugation.
The key benefit of asymmetric flow field-flow fractionation (AF4) over conventional SEC column chromatography is the capability to separate both colloidal and soluble components over a wide size range, from 1nm to 1000nm.
The key features of the Eclipse™AF4 Asymmetric-Flow Field-Flow Fractionation System are:
- The AF4 uses an open separation channel without any stationary phase and minimal surface area, and therefore generates almost no shear that can degrade sensitive samples.
- As minimal surface area is involved, adverse interactions between the sample and the surface are reduced relative to sizing columns.
- Its resolution is almost the same as analytical ultracentifugation, however it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.
- The Eclipse AF4 can be seamlessly combined with various market-leading HPLC packages, such as Agilent, Thermo Scientific and Shimadzu, using high-quality pumps, degassers, autosamplers and UV detectors while replacing just the column with an AF4 channel.
- The chassis can be ordered in three versions: for aqueous solvents (biocompatible), for organic solvents and in a metal-free version.
- For higher versatility consider the Eclipse DualTec, which supports automatic switching between AF4 and SEC as well as the hollow-fiber field-flow fractionation channel for nL-scale separations.