Eclipse™AF4 Asymmetric-Flow Field-Flow Fractionation System from Wyatt Technology

The Eclipse™AF4 is used for challenging fractionation, sizing and separation tasks for naoparticles, macromolecules and colloids. In combination with a Wyatt DAWN® or miniDAWN™ MALS detector and Optilab® T-rEX™ refractive index detector, the Eclipse AF4 provides a versatile solution offering characterization of absolute molar mass and size and also conformation and conjugation.

The key benefit of asymmetric flow field-flow fractionation (AF4) over conventional SEC column chromatography is the capability to separate both colloidal and soluble components over a wide size range, from 1nm to 1000nm.

Key Features

The key features of the Eclipse™AF4 Asymmetric-Flow Field-Flow Fractionation System are:

  • The AF4 uses an open separation channel without any stationary phase and minimal surface area, and therefore generates almost no shear that can degrade sensitive samples.
  • As minimal surface area is involved, adverse interactions between the sample and the surface are reduced relative to sizing columns.
  • Its resolution is almost the same as analytical ultracentifugation, however it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.
  • The Eclipse AF4 can be seamlessly combined with various market-leading HPLC packages, such as Agilent, Thermo Scientific and Shimadzu, using high-quality pumps, degassers, autosamplers and UV detectors while replacing just the column with an AF4 channel.
  • The chassis can be ordered in three versions: for aqueous solvents (biocompatible), for organic solvents and in a metal-free version.
  • For higher versatility consider the Eclipse DualTec, which supports automatic switching between AF4 and SEC as well as the hollow-fiber field-flow fractionation channel for nL-scale separations.
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