Litesizer Series of Particle Size Analyzers

The Litesizer series from Anton is the ideal choice for characterizing microparticles and nanoparticles just by touching a button. The particles present in dispersion as well as macromolecules or peptides in solution can be identified over a wide concentration range, which reduces the time taken for preparing samples.

Litesizer 500 consists of three measurement angles for particle size determination as well as patented cmPALS technology, allowing zeta potential measurements at low voltages with the highest repeatability and precision.

Litesizer 500

  • Particle size measurements can be executed through dynamic light scattering at three different measurement angles
  • Particle characterization performed from the nano- to the micrometer range
  • Molecular mass and refractive index measurements
  • Zeta potential is determined using patented cmPALS

Litesizer 100

  • Particle size measurements range from the nano- to the micrometer range
  • Constant transmittance measurement
  • Automatic measurement modes are available
  • DLS back scatter detection guarantees wide concentration range

Key Features

Litesizer Chooses the Ideal Measurement Angle for Users’ Sample to Ensure the Highest Data Quality

Three detection angles are available for DLS measurements in Litesizer 500 (side-, back- or forward scattering). This enables optimal parameter settings. With the help of the integrated automatic angle selection, the instrument can specify the most appropriate angle for users’ samples.

The Litesizer 100 makes use of the versatile backscatter angle, which is perfect for turbid samples since it decreases multiple scattering events and allows optimal data quality. Users can get a complete analysis of their sample as the Litesizer series has the ability to resolve up to three distinct particle size classes in a single measurement.

Litesizer Series of Particle Size Analyzers

Image Credit: Anton Paar GmbH

Zeta Potential Measurements Down to 0.1 mg/mL for the Highest Sensitivity

Litesizer 500 enables users to execute zeta potential measurements through electrophoretic light scattering. The patented cmPALS method (European Patent 2 735 870) allows highly sensitive measurements to be performed for useful findings even at the lowest sample concentration, in considerably shorter measuring times and with the least sample degradation.

Particularly, this is useful for peptide solutions. Furthermore, the special Anton Paar Ω-shaped capillary of the zeta potential cuvettes makes a stable electric field precisely at the measurement position, leading to highly reproducible zeta potential measurements.

Litesizer Series of Particle Size Analyzers

Image Credit: Anton Paar GmbH

Continuous Transmittance Measurement for Sample Monitoring

The constantly measured transmittance provides users with instant feedback regarding their sample. Moreover, it enables automatic optimization of measurement parameters like focus position, measurement angle and duration. Also, the transmittance offers data about the start of aggregation or sedimentation in a series measurement.

Litesizer Series of Particle Size Analyzers

Image Credit: Anton Paar GmbH

All-in-One Instrument — Determination of the Refractive Index

The refractive index of the sample solvent is an essential input parameter for DLS and ELS measurements. Litesizer 500 has the ability to quantify the refractive index at the exact temperature and wavelength of users’ DLS or ELS measurement (European Patent 3 023 770), while for a majority of the particle analyzers, these indices should be found from external sources.

This guarantees maximum precision of particle size and zeta potential findings under all experimental conditions. Litesizer 500 finds out the refractive index below ±0.5 %, as specified by ISO 22412:2017 regarding the precision of the refractive index needed for DLS.

Litesizer Series of Particle Size Analyzers

Image Credit: Anton Paar GmbH

Get a Clear Overview of Users’ Measurements with the Kalliope Software

The measurement software called Kalliope from the Litesizer series offers an intuitive one-page workflow. Measurement signals, input parameters and results are all at hand. Also, the one-page workflow enables users to commence a measurement with just three clicks.

Pre-set standard reports and structured result overviews offer quick access to the measurement result. Users can also have an in-depth look into the data through the Excel export, analysis function and tailored report templates. Kalliope is completely compliant with the FDA’s 21 CFR Part 11 regulations.

Litesizer Series of Particle Size Analyzers

Image Credit: Anton Paar GmbH

Technical Specifications

Source: Anton Paar GmbH

  Litesizer 500 Litesizer 100
Particle size specifications
Measurement principle Dynamic Light Scattering (DLS)
Measuring range 0.3 nm to 10 μm (particle diameter)
Measurement angles 15°, 90°, 175° 175°
Min. concentration 0.1 mg/mL (lysozyme)
Max. concentration 50% w/v (sample-dependent)
Min. sample volume 12 µL
Accuracy Better than +/-2% on NIST traceable standards
Repeatability Better than +/-2% on NIST traceable standards
Zeta potential specifications
Measuring principle Electrophoretic Light Scattering (ELS) / cmPALS -
Measuring range >= +/-1000 mV -
Mobility range 10 -11 m²/V.s to 2 x 10 -7 m²/V.s -
Size range 3.8 nm to 100 µm (diameter) -
Min. sample concentration 0.1 mg/mL (lysozyme) -
Max. sample concentration 70% w/v (sample-dependent) -
Max. sample conductivity 200 mS/cm -
Min. sample volume 50 µL (sample-viscosity-dependent) -
Accuracy Better than +/-10% -
Repeatability +/-3% -
Molecular mass specifications
Measuring principle Static Light Scattering (SLS) -
Measuring range (mass) 980 Da to 20 MDa -
Measuring range (particle size) up to 40 nm (diameter) -
Measurement angle 90° -
Min. sample concentration 0.1 mg/mL (lysozyme) -
Accuracy +/-10% -
Repeatability +/-5% -
Transmittance specifications
Measuring range 0% to 100%
Min. sample volume 15 µL
Accuracy Better than +/-1%
Refractive index specifications
Measuring range 1.28 to 1.50 -
Min. sample volume 1 mL -
Accuracy +/-0.5% -
General specifications
Light source Semiconductor laser diode / 40 mW, 658 nm
Laser warm-up time 6 min
Temperature control range 0 °C to 90 °C
Ambient operating temperature 10 °C to 35 °C
Humidity up to 80% non-condensing
Dimensions (W, D, H) 460 mm, 485 mm, 135 mm
Weight approx. 18 kg (40 lb) approx. 16.3 kg (36 lb)

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