The study was conducted on mice by two members of Di Polo's lab: Dr. Luis Alarcon-Martinez, a postdoctoral fellow, and Deborah Villafranca-Baughman, a PhD student. Both are the first co-authors of this study.
In living animals, as in humans, the retina uses the oxygen and nutrients contained in the blood to fully function. This vital exchange takes place through capillaries, the thinnest blood vessels in all organs of the body. When the blood supply is dramatically reduced or cut off--such as in ischemia or stroke--the retina does not receive the oxygen it needs. In this condition, the cells begin to die and the retina stops working as it should.
Tunnelling between cells
Wrapped around the capillaries are pericytes, cells that have the ability to control the amount of blood passing through a single capillary simply by squeezing and releasing it.
"Using a microscopy technique to visualize vascular changes in living mice, we showed that pericytes project very thin tubes, called inter-pericyte tunnelling nanotubes, to communicate with other pericytes located in distant capillaries," said Alarcon-Martinez. "Through these nanotubes, the pericytes can talk to each other to deliver blood where it is most needed."
Another important feature, added Villafranca-Baughman, is that "the capillaries lose their ability to shuttle blood where it is required when the tunnelling nanotubes are damaged--after an ischemic stroke, for example. The lack of blood supply that follows has a detrimental effect on neurons and the overall tissue function."
The team's findings suggest that microvascular deficits observed in neurodegenerative diseases like strokes, glaucoma, and Alzheimer's disease might result from the loss of tunnelling nanotubes and impaired blood distribution. Strategies that protect these nanostructures should then be beneficial, but remain to be demonstrated.
This research was supported by the Canadian Institutes of Health Research.
Further reading: "Inter-pericyte tunnelling nanotubes regulate neurovascular coupling" by Luis Alarcon-Martinez, Deborah Villafranca-Baughman et al. in Nature DOI: 10.1038/s41586-020-2589-x