AnaSpec has introduced the newest member of the HiLyte Fluor™ family - HiLyte Fluor™ 594 (Ex/Em=593/616 nm), a novel bright and red fluorescent probe.
HiLyte Fluor™ dyes are a series of high-performance fluorescent labeling dyes that span the full visible spectrum. These dyes have been optimized to yield maximum brightness when conjugated with macromolecules.
Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives - allowing easy conjugation to biopolymers. HiLyte Fluor™ 594 has spectral characteristics similar to those of Texas Red® (a trade mark of Invitrogen for sulforhodamine 101 sulfonyl chloride), with excitation and emission wavelength at 593/615 nm in PBS buffer (pH 7.4), and 596/617 nm when conjugated to macromolecules. It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen). The fluorescence quantum yield of HiLyte Fluor™ 594 is 0.9 in aqueous solution. HiLyte Fluor™ 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells. Bioconjugates conjugated to HiLyte Fluor™ 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.
HiLyte Fluor™ 594 conjugated proteins have been optimized in a specific fluorophore / protein labeling ratio to ensure a high fluorescent signal and uncompromised protein functions. The HiLyte Fluor™ 594-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications.