Nanobubbles Becoming the Subject of an Increasing Amount of Study

Despite initial scepticism, acceptance of the existence and special properties of nanobubbles is now growing and their formation and characteristics are now becoming the subject of an increasing amount of study, especially in Japan. NanoSight proves to be the method of choice for their study.

Due to the supposed very high pressure within bubbles of such small size and radius of curvature and thus high surface tension, conventional calculations show that the gas should be ‘pressed out’ of the nanobubbles within microseconds. However, it is now clear that under the right conditions such bubbles can both form freely and remain stable of extended time periods, sometimes many months. Explanations as to just why such structures are so stable are focussing on the role of counter-ions forming layers at the nanobubbles surface, which helps explain claims that they apparently form only in the presence of salts.

Kaneo Chiba and Masayoshi Takahashi of Japan’s famous AIST research centre have shown that in the presence of electrolytes and with the correct physical stimulus, stable nanobubbles can be formed from conventional microbubbles. The latter tend to either to coalesce to large buoyant bubbles which float away or which collapse under intense surface tension-derived pressure to the point they vanish as predicted by theory. The addition of salt (electrolytes) however, is thought to cause the formation of a counter-ion screen around nanobubbles which effectively blocks the ability of gases within the nanobubbles to diffuse out. This was confirmed by electrophoresis studies in which the zeta potential of nanobubbles was shown to be related to nanobubbles stability.

Furthermore, Professor William Drucker of the University of Melbourne has also used infra-red spectroscopy to show that the pressure of gas within such nanobubbles is not significantly higher than atmospheric, perhaps explaining their stability and resistance to collapse.

There exists a wide range of proposed applications of nanobubbles and interest in their usage is growing rapidly. When formed from ozone and electrolyte stabilised, disinfection and sterilisation is possible for many months with great potential in the preservation of foodstuffs and in medical applications as an attractive alternative to chlorine based methodologies.

Oxygen nanobubbles have been implicated in the prevention of arteriosclerosis by the inhibition of mRNA expression induced by cytokine stimulation in rat aorta cell lines.

When formed in liquids in capillaries, nanobubbles have been shown to greatly improve liquid flow characteristics. They have also been proposed as contrast agents in scanning techniques as well as cleaning agents in silicon manufacturing processes.

Finally, a new field of drug delivery applications is being actively researched in which nanobubbles play an active part though details of this highly secretive (and therefore important?) field are hard to come by. However, reported in Reuters, Natalya Rapoport of the University of Utah's Department of Bioengineering is using nanobubbles with the chemotherapy drug doxorubicin seek out cancer tumors and congregate when injected into the bloodstream. "These nanobubbles don't penetrate normal blood vessels but they do penetrate blood vessels in the tumor," said Rapoport, whose study appears in the Journal of the National Cancer Institute. Once in the tumor, the nanobubbles combine to form larger "microbubbles," which can be seen on an ultrasound. "When these bubbles accumulate, I give strong ultrasound radiation to the tumor to blow them up," she said in a telephone interview. "Then the drug gets out of these bubbles locally at the tumor site."

In mice, the nanobubbles were more effective at blocking tumor growth than other nanoparticle delivery methods.

In a previous study, Kikuchi et al (2001) showed that hydrogen nanobubbles formation was related to the influence of electrolysis conditions on the hydrogen content and the diameter distribution of hydrogen nanobubbles. They analysed the nanobubbles by Dynamic Light Scattering (DLS). However, in a recent study of the formation and characterisation of nanobubbles in water by a major pharmaceutical company in Japan, the concentration of nanobubbles in a mechanically formed suspension of nanobubbles was found to be very low (<107/ml), a concentration which would be too low for meaningful analysis of DLS. Of course, analysis by Electron Microscopy was not possible because of the vacuum required for EM studies. NanoSight was shown to be ideally suited to such analyses and in a blind experiment, in which three samples of nanobubbles suspensions containing high, low and zero numbers of nanobubbles were tested in duplicate, NanoSight results were found to match exactly those predicted. The graph below shows the results in which sample type A (Series 1 and 6 ) contained high concentration of nanobubbles, sample B (Series 3 and 5) contained low concentration of nanobubbles and sample C (Series 2 and 4) were control blanks. It should be noted that NanoSight allows concentration of nanobubbles per unit volume to be estimated as well as size and size distribution.

In a more recent project, Ichiro Otsuka (2008) of Ohu University, Japan has studied the possible role of nanobubbles in ultra-high diluted samples of active agents in which the phenomenon of succussion is considered to relevant. He used NanoSight technology to examine nanobubbles formation and concentration in more detail than was possible using an electrozone (Coulter) method or conventional DLS.

Kenji Kikuchi, Hiroko Takeda, Beatrice Rabolt, Takuji Okaya, Zempachi Ogumi, Yasuhiro Saihara and Hiroyuki Noguchi (2001) Hydrogen particles and supersaturation in alkaline water from analkali–ion–water electrolyzer, Journal of Electroanalytical Chemistry, p1–6

Ichiro Otsuka (2008) Effect of 1:2 aqueous dilution on O2 nanobubbles in a 0.1 M Na2CO3 solution, Proc The 59th Annual Meeting of the International Society of Electrochemistry, September 7 to 12, 2008, Seville, Spain; s10-P-062, p139

Tell Us What You Think

Do you have a review, update or anything you would like to add to this news story?

Leave your feedback
Your comment type

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.