Posted in | News | Nanolithography

AFM Nanolithography for Single Chromosome Nanomanipulation to Generate Single-Cell Genetic Probes

Chromosomal dissection enables separation of DNA from cytogenetically identified region to produce genetic probes for exhibiting fluorescence in situ hybridization, a method that is popular in cyto and molecular genetics study and diagnostics.

AFM

Several reports are available that provide information regarding methods of microdissection such as laser beam or glass needle to acquire specific probes from metaphase chromosomes.

The traditional dissection methods tend to have many disadvantages as it requires numerous chromosomes for manufacturing a probe. Also, due to the large size of microneedles, the traditional methods are not apt for single chromosome analysis. Hence it is important to have new dissection techniques that can provide advancement in chromosomal research at the nano level.

The atomic force microscope (AFM) is used as a tool for single chromosomes nanomanipulation to produce single genetic probes that target particular cells. This method integrates nanomanipulation and molecular techniques to enable amplification and nanodissection of chromatidial and chromosomal DNA.

A cross-sectional examination of dissected chromosomes displays 20 nm and 40 nm deep incisions. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) is applied to amplify and label stand-alone, individual chromosomal portions and later to hybridize to interphasic nuclei and chromosomes.

Atomic force microscope can be used to view and control biological substances with high precision and resolution. The fluorescence in situ hybridization (FISH) carried out with DOP-PCR devices as test probes have been effectively experimented in interphasic nuclei and avian microchromosomes.

Chromosome nanolithography has a resolution higher than that of light microscopy. It can be helpful for constructing chromosome band libraries and mapping of the molecular cytogenetic mapping used for study of genetic diseases.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Chai, Cameron. (2019, February 12). AFM Nanolithography for Single Chromosome Nanomanipulation to Generate Single-Cell Genetic Probes. AZoNano. Retrieved on October 08, 2024 from https://www.azonano.com/news.aspx?newsID=22823.

  • MLA

    Chai, Cameron. "AFM Nanolithography for Single Chromosome Nanomanipulation to Generate Single-Cell Genetic Probes". AZoNano. 08 October 2024. <https://www.azonano.com/news.aspx?newsID=22823>.

  • Chicago

    Chai, Cameron. "AFM Nanolithography for Single Chromosome Nanomanipulation to Generate Single-Cell Genetic Probes". AZoNano. https://www.azonano.com/news.aspx?newsID=22823. (accessed October 08, 2024).

  • Harvard

    Chai, Cameron. 2019. AFM Nanolithography for Single Chromosome Nanomanipulation to Generate Single-Cell Genetic Probes. AZoNano, viewed 08 October 2024, https://www.azonano.com/news.aspx?newsID=22823.

Tell Us What You Think

Do you have a review, update or anything you would like to add to this news story?

Leave your feedback
Your comment type
Submit

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.