EM AFS2 supports freeze substitution and progressive lowering of temperature (PLT) workflows for low-temperature embedding and resin polymerization in light and electron microscopy. Optional automation features (e.g., reagent handling and in-chamber visibility) help reduce hands-on steps while running protocols.
Key Features
- Streamline freeze substitution and PLT in one workflow to support low-temperature embedding and resin polymerization for LM and EM applications.
- Reduce reagent handling and shorten setup through automatic dilution from 100% stock containers (with the EM FSP configuration).
- Cut manual solution exchange steps when configured for automation: the Leica EM FSP dispenses reagents for freeze substitution and PLT applications.
- Enable “load-and-run” UV embedding workflows with fast UV polymerization via an LED UV lamp, allowing samples and reagents to be added and left until the end of the run.
- Help prevent moisture-related contamination during low-temperature processing with an adjustable gaseous LN2 transfer function that keeps chamber and specimens dry.
- Improve handling confidence inside the chamber using internal LED illumination and an attached stereomicroscope for viewing and positioning samples.