Researchers from BioSeek and the U.S. Environmental Protection Agency (EPA) have presented the evaluations of the biological activity of different chemicals and nanomaterials in the background of primary human cell biology at the Society of Toxicology’s annual meeting.
The presented assessments corroborate the importance of BioSeek’s BioMAP human primary cell assay systems for determining key bioactivities and potentially harmful effects of new materials, drugs and other compounds in high-output formats.
In EPA’s oral presentation, cytotoxicity of different nanomaterials with changeable cores, and their micro and ion counterparts were tested in different types of cells for bioactive profiling of proteins in 8 BioMAP human primary cell systems and for transcription factor activation in HepG2cells at a concentration level comparable to human being’s round-the-clock exposure to 45 years. Analysis demonstrated that nanomaterial cores are vital to bioactivities and their impacts are often the same as that of their ion counterparts. The comparison of the test findings on nanomaterials with other compounds’ reference profiles in the BioMAP database showed the impact on more molecular pathways and targets by the tested nanomaterials, which were not measured directly by the assays conducted.
A poster presented by the researchers from BioSeek and EPA described the ToxCast Phase II Chemical Library biological profiling in the primary human cell co-culture systems of Bioseek. The Phase II library comprises 1060 distinctive compounds including developmental/ reproductive toxicants or carcinogens, reference compounds called as endocrine disrupters, failed drugs contributed by industry partners, proposed replacements to existing industrial chemicals, cosmetic and food additives, and other broadly utilized chemicals.
The chemicals were analyzed in BioMAP co-culture systems and categorized on the basis of their bioactivity profiles in comparison with reference compounds and their capability to cause cytotoxicity obviously in different types of cells.
Source: http://www.bioseekinc.com