Dynamics of CM15 Antimicrobial Activity on Outer Membrane of E. coli Cells

This video shows the CM15 antimicrobial activity on the nanoscale structure of E. coli bacterial cell membrane. The images were captured every 8s at a resolution of 1024 x 256 pixels using Dimension FastScan Bio AFM (atomic force microscopy) from Bruker Nano Surfaces.

Every second image in the time series is shown after ~96s incubation when the effects of CM15 were first observed as an increase in membrane roughness (corresponding graph shown above), accompanied by the disappearance of the ordered porin structures, membrane micellization, and the appearance of “pore-like” lesions in the membrane surface.

Run time – 0:06sec

AFM Bio | CM15 Antimicrobial Activity on Nanoscale Structure of E. coli Bacterial Membrane | Bruker

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